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Chem Commun (Camb). 2011 Jan 7;47(1):397-9. doi: 10.1039/c0cc02229b. Epub 2010 Sep 9.

A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death.

Author information

1
Department of Chemistry and Biochemistry, University of Arizona, 1306 East University Blvd, Tucson, AZ 85721, USA.

Abstract

Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.

PMID:
20830433
DOI:
10.1039/c0cc02229b
[Indexed for MEDLINE]

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