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Infect Immun. 2010 Nov;78(11):4773-8. doi: 10.1128/IAI.00567-10. Epub 2010 Sep 7.

Fusobacterium nucleatum outer membrane proteins Fap2 and RadD induce cell death in human lymphocytes.

Author information

1
Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.

Abstract

Bacterially induced cell death in human lymphocytes is an important virulence factor for pathogenic bacteria. Previously discovered mechanisms of bacterially induced cell death are predominantly based on the transfer of bacterial proteins to the target host cell, such as the toxins secreted through type I, II, and VI secretion systems or effector proteins injected through type III, IV, and Vb secretion systems. Here, we report a mechanism employed by the Gram-negative oral pathogen Fusobacterium nucleatum for cell death induction of human lymphocytes via two outer membrane proteins (OMPs), Fap2 and RadD, which share regions homologous to autotransporter secretion systems (type Va secretion systems). Genetic and physiological studies established that inactivation of the two OMPs led to significantly reduced ability to trigger cell death in Jurkat cells, while the corresponding double mutant was almost completely attenuated. Additional biochemical and molecular analyses demonstrated that cell-free F. nucleatum membranes are sufficient to induce cell death in Jurkat cells, suggesting that no active process or effector protein transfer was necessary to induce eukaryotic cell death.

PMID:
20823215
PMCID:
PMC2976331
DOI:
10.1128/IAI.00567-10
[Indexed for MEDLINE]
Free PMC Article

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