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Vet Pathol. 2011 Jul;48(4):834-8. doi: 10.1177/0300985810380399. Epub 2010 Sep 3.

Efficient and cost-effective extraction of genomic DNA from formalin-fixed and paraffin-embedded tissues.

Author information

1
Department of Veterinary Pathology, Freie Universit├Ąt Berlin, Robert-von-Ostertag-Str. 15, 14163 Berlin, Germany. weiss.alexander@vetmed.fu-berlin.de

Abstract

Diagnostic and investigative molecular pathology frequently has to resort to extraction of DNA from formalin-fixed and paraffin-embedded tissue samples. Although many different protocols are reported for this type of material, extraction of sufficient amounts of intact DNA is still challenging. Here, the authors report a reproducible, simple, cost-effective, and efficient protocol that yields up to 140 ╬╝g of DNA from approximately 10 to 15 mg of formalin-fixed and paraffin-embedded tissue samples and compare it to available protocols. The protocol allows stable amplification of DNA fragments up to 600 bp in length in a wide variety of tissues.

PMID:
20817894
DOI:
10.1177/0300985810380399
[Indexed for MEDLINE]

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