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Methods Enzymol. 2010;479:73-91. doi: 10.1016/S0076-6879(10)79004-2.

Transcript analysis of stem cells.

Author information

1
The Complex Carbohydrate Research Center and the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.

Abstract

Quantitative real-time polymerase chain reaction (qRT-PCR) is a flexible and scalable method for analyzing transcript abundance that can be used at a single gene or high-throughput (>100 genes) level. Information obtained from this technique can be used as an indicator of potential regulation of glycosylation at the transcript level when combined with glycan structural or protein abundance data. This chapter describes detailed methods to design and perform qRT-PCR analyses and provides examples of information that can be obtained from the technique.

PMID:
20816160
PMCID:
PMC3104320
DOI:
10.1016/S0076-6879(10)79004-2
[Indexed for MEDLINE]
Free PMC Article

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