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Cell Metab. 2010 Sep 8;12(3):260-72. doi: 10.1016/j.cmet.2010.08.004.

Insulin-like signaling determines survival during stress via posttranscriptional mechanisms in C. elegans.

Author information

1
The Mental Health Research Institute, Parkville, Victoria, Australia. gmccoll@mhri.edu.au

Abstract

The insulin-like signaling (ILS) pathway regulates metabolism and is known to modulate adult life span in C. elegans. Altered stress responses and resistance to a wide range of stressors are also associated with changes in ILS and contribute to enhanced longevity. The transcription factors DAF-16 and HSF-1 are key effectors of the longevity phenotype. We demonstrate that increased intrinsic thermotolerance, due to lower ILS, is not dependent on stress-induced transcriptional responses but instead requires active protein translation. Translation profiling experiments reveal genes that are posttranscriptionally regulated in response to altered ILS during heat shock in a DAF-16-dependent manner. Furthermore, several novel proteins are specifically required for ILS effects on thermotolerance. We propose that lowered ILS results in metabolic and physiological changes. These DAF-16-induced changes precondition a translational response under acute stress to modulate survival.

PMID:
20816092
PMCID:
PMC2945254
DOI:
10.1016/j.cmet.2010.08.004
[Indexed for MEDLINE]
Free PMC Article

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