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Rev Sci Instrum. 2010 Aug;81(8):084301. doi: 10.1063/1.3473937.

Microbial population dynamics by digital in-line holographic microscopy.

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1
Laboratory of Living Matter, The Rockefeller University, 1230 York Avenue, New York, New York 10065, USA.

Abstract

Measurements of population dynamics are ubiquitous in experiments with microorganisms. Studies with microbes elucidating adaptation, selection, and competition rely on measurements of changing populations in time. Despite this importance, quantitative methods for measuring population dynamics microscopically, with high time resolution, across many replicates remain limited. Here we present a new noninvasive method to precisely measure microbial spatiotemporal population dynamics based on digital in-line holographic (DIH) microscopy. Our inexpensive, replicate DIH microscopes imaged hundreds of swimming algae in three dimensions within a volume of several microliters on a time scale of minutes over periods of weeks.

PMID:
20815617
PMCID:
PMC2966488
DOI:
10.1063/1.3473937
[Indexed for MEDLINE]
Free PMC Article
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