Studies have shown that high concentrations of total cholesterol at the expense of LDL cholesterol and markers of inflammation are linked with subclinical atherosclerosis. This study was aimed to associate the carotid intima media thickness (IMT) and high-sensitivity C-reactive protein (hs-CRP) in patients with isolated hypercholesterolemia. The investigation was descriptive, transverse and included 100 patients divided into a control group (normocholesterolemic) and a study group (hypercholesterolemic). There were no significant differences in age and gender distribution among the groups. The body mass index, waist circumference, total cholesterol and its fractions, IMT and hs-CRP were significantly elevated in the study group. Only in the hypercholesterolemic group, the IMT was significantly associated with hsCRP. In the study group, the mean IMT rose by quartiles of hs-CRP, reaching its highest level in the fourth quartile. The multiple linear regression analysis identified LDL cholesterol and hs-CRP as predictors of IMT only in the study group. The findings support other data suggesting that not only the LDL cholesterol but also hs-CRP could be an important factor for early atherosclerotic changes in the carotid artery of patients with isolated hypercholesterolemia.