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Anal Bioanal Chem. 2011 Jan;399(2):527-39. doi: 10.1007/s00216-010-4121-x. Epub 2010 Sep 3.

Analysis and characterization of heparin impurities.

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1
Department of Chemistry, University of California, Riverside, CA 92521, USA.

Abstract

This review discusses recent developments in analytical methods available for the sensitive separation, detection and structural characterization of heparin contaminants. The adulteration of raw heparin with oversulfated chondroitin sulfate (OSCS) in 2007-2008 spawned a global crisis resulting in extensive revisions to the pharmacopeia monographs on heparin and prompting the FDA to recommend the development of additional physicochemical methods for the analysis of heparin purity. The analytical chemistry community quickly responded to this challenge, developing a wide variety of innovative approaches, several of which are reported in this special issue. This review provides an overview of methods of heparin isolation and digestion, discusses known heparin contaminants, including OSCS, and summarizes recent publications on heparin impurity analysis using sensors, near-IR, Raman, and NMR spectroscopy, as well as electrophoretic and chromatographic separations.

PMID:
20814668
PMCID:
PMC3015169
DOI:
10.1007/s00216-010-4121-x
[Indexed for MEDLINE]
Free PMC Article

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