Format

Send to

Choose Destination
EMBO Rep. 2010 Oct;11(10):772-6. doi: 10.1038/embor.2010.124. Epub 2010 Sep 3.

A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes.

Author information

1
Abteilung für molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, Göttingen 37077, Germany.

Abstract

Despite the fundamental role of canonical histones in nucleosome structure, there is no experimental system for higher eukaryotes in which basic questions about histone function can be directly addressed. We developed a new genetic tool for Drosophila melanogaster in which the canonical histone complement can be replaced with multiple copies of experimentally modified histone transgenes. This new histone-replacement system provides a well-defined and direct cellular assay system for histone function with which to critically test models in chromatin biology dealing with chromatin assembly, variant histone functions and the biological significance of distinct histone modifications in a multicellular organism.

PMID:
20814422
PMCID:
PMC2948182
DOI:
10.1038/embor.2010.124
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center