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Eur J Immunol. 2010 Oct;40(10):2882-90. doi: 10.1002/eji.201040422.

Chitin induces upregulation of B7-H1 on macrophages and inhibits T-cell proliferation.

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1
Institute for Immunology, Medical Faculty, University of Munich, Munich, Germany.

Abstract

Chitin is a highly abundant glycopolymer, which serves as structural component in fungi, arthropods and crustaceans but is not synthesized by vertebrates. However, vertebrates express chitinases and chitinase-like proteins, some of which are induced by infection with helminths suggesting that chitinous structures may be targets of the immune system. The chitin-induced modulations of the innate and adaptive immune responses are not well understood. Here, we demonstrate that intranasal administration of OVA and chitin resulted in diminished T-cell expansion and Th2 polarization as compared with OVA administration alone. Chitin did not promote nor attenuate Th2 polarization in vitro. Chitin-exposed macrophages inhibited proliferation of CD4(+) T cells in a cell-cell contact-dependent manner. Chitin induced upregulation of the inhibitory ligand B7-H1 (PD-L1) on macrophages independently of MyD88, TRIF, TLR2, TLR3, TLR4 and Stat6. Inhibition of T-cell proliferation was largely dependent on B7-H1, as the effect was not observed in cocultures with cells from B7-H1-deficient mice.

PMID:
20812235
DOI:
10.1002/eji.201040422
[Indexed for MEDLINE]
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