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J Microbiol Methods. 2010 Nov;83(2):185-7. doi: 10.1016/j.mimet.2010.08.014. Epub 2010 Aug 31.

Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system.

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Department of Microbiology, Laikon General Hospital, Athens, Greece.


hyplex®-MBL ID Multiplex PCR-ELISA, a novel method for identifying metallo-β-lactamase genes directly in clinical specimens, was evaluated using a consecutive collection of 326 samples from three hospitals in Greece characterized by high prevalence of VIM producers. The method exhibited high sensitivity (98.0%) and specificity (98.6%) and was proven reliable in detecting bla(VIM) genes in blood, urine, pus, and sputum samples that, as confirmed by conventional methods, contained various VIM-producing species. Future multicenter studies should be considered for the thorough evaluation of this method and its potential diagnostic utility.

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