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J Magn Reson. 2010 Nov;207(1):164-7. doi: 10.1016/j.jmr.2010.08.002. Epub 2010 Aug 11.

EPR distance measurements in deuterated proteins.

Author information

1
School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.

Abstract

One of the major problems facing distance determination by pulsed EPR, on spin-labeled proteins, has been the short relaxation time T(m). Solvent deuteration has previously been used to slow relaxation and so extend the range of distance measurement and sensitivity. We demonstrate here that deuteration of the underlying protein, as well as the solvent, extends the T(m) to a considerable degree. Longer T(m) gives greatly enhanced sensitivity, much extended distance measurement, more reliable distance distribution calculation and better baseline correction.

PMID:
20805036
PMCID:
PMC3516800
DOI:
10.1016/j.jmr.2010.08.002
[Indexed for MEDLINE]
Free PMC Article

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