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Clin Biochem. 2010 Nov;43(16-17):1368-70. doi: 10.1016/j.clinbiochem.2010.08.020. Epub 2010 Aug 25.

Time of sampling is crucial for measurement of cell-free plasma DNA following acute aseptic inflammation induced by exercise.

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Department of Physical Education and Sports Science, Democritus University of Thrace, Komotini 69100, Greece.



To determine the time-course changes of cell-free plasma DNA (cfDNA) following heavy exercise.


cfDNA concentration, C-reactive protein levels (hs-CRP), uric acid concentration (UA), creatine kinase activity (CK) were measured before and post-exercise (immediately post, 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, 8h, 10h, 24h).


cfDNA increased (15-fold) 30-min post-exercise and normalized thereafter. hs-CRP increased (56%, p<0.001) 1h post-exercise, remained elevated throughout recovery (52-142%, p<0.0001), and peaked (200% rise, p<0.0001) at 24h post-exercise. UA and CK increased (p<0.05), immediately post-exercise, remained elevated throughout recovery (p<0.0001), and peaked (p<0.0001) at 24h of post-exercise recovery.


cfDNA sampling timing is crucial and a potential source of error following aseptic inflammation.

[Indexed for MEDLINE]

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