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J Am Chem Soc. 2010 Sep 22;132(37):12780-1. doi: 10.1021/ja104299t.

Quantum-dot-decorated robust transductable bioluminescent nanocapsules.

Author information

1
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, California 90095, USA.

Abstract

Bioluminescence, due to its high sensitivity, has been exploited in various analytical and imaging applications. In this work, we report a highly stable, cell-transductable, and wavelength-tunable bioluminescence system achieved with an elegant and simple design. Using aqueous in situ polymerization on a bioluminescent enzyme anchored with polymerizable vinyl groups, we obtained nanosized core-shell nanocapsules with the enzyme as the core and a cross-linked thin polymer net as the shell. These nanocapsules possess greatly enhanced stability, retained bioactivity, and a readily engineered surface. In particular, by incorporating polymerizable amines in the polymerization, we endowed the nanocapsules with efficient cell-transduction and sufficient conjugation sites for follow-up modification. Following in situ polymerization, decorating the polymer shell with fluorescent quantum dots allowed us to access a continuous tunable wavelength, which extends the application of such bioluminescent nanocapsules, especially in deep tissue. In addition, the unique core-shell structure and adequate conjugation sites on surface enabled us to maximize the BRET efficiency by adjusting the QD/enzyme conjugation ratio.

PMID:
20795619
PMCID:
PMC2976844
DOI:
10.1021/ja104299t
[Indexed for MEDLINE]
Free PMC Article

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