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Biol Chem Hoppe Seyler. 1990 Oct;371(10):929-38.

Non-radioactive labeling and detection of nucleic acids. II. Optimization of the digoxigenin system.

Author information

1
Boehringer Mannheim GmbH, Biochemisches Forschungszentrum Penzberg, Tutzing.

Abstract

The random-primed DNA labeling technique was modified for the incorporation of digoxigenin into DNA as basic component of the digoxigenin-based non-radioactive DNA labeling and detection system. Digoxigenin molecules act as reporter groups for highly sensitive DNA detection by a digoxigenin-specific antibody:alkaline phosphatase-conjugate-catalysed color reaction. The parameters affecting the individual reaction steps of the digoxigenin labeling, hybridization and detection reactions were optimized to maximal sensitivity and specificity.

PMID:
2076200
DOI:
10.1515/bchm3.1990.371.2.929
[Indexed for MEDLINE]

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