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Methods Mol Biol. 2010;655:413-32. doi: 10.1007/978-1-60761-765-5_28.

Immunocytological analysis of chromatin in isolated nuclei.

Author information

1
Laboratory of Genetics, Wageningen University, Wageningen, Netherlands.

Abstract

All cells in a multicellular organism have the same genetic constitution, yet their appearance and function may differ enormously, due to differences in the nuclear program. Central in the establishment of this cell diversity are epigenetic marks, which are largely based on covalent modifications of histones and methylated cytosine residues in the DNA sequence. The study of these epigenetic factors in individual cells requires the microscopic visualization of chromatin components. Here we describe a number of protocols to study chromatin in isolated nuclei.

PMID:
20734277
DOI:
10.1007/978-1-60761-765-5_28
[Indexed for MEDLINE]

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