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Nat Methods. 2010 Sep;7(9):721-3. doi: 10.1038/nmeth.1494. Epub 2010 Aug 22.

An antibiotic selection marker for nematode transgenesis.

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1
Genome Regulation and Evolution, INSERM U869, Institut Européen de Chimie et Biologie, Pessac, France.

Abstract

We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).

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PMID:
20729841
DOI:
10.1038/nmeth.1494
[Indexed for MEDLINE]

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