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Nat Cell Biol. 2010 Sep;12(9):902-8. doi: 10.1038/ncb2094. Epub 2010 Aug 22.

Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system.

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1
Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA.

Erratum in

  • Nat Cell Biol. 2010 Oct;12(10):1021.
  • Nat Cell Biol. 2010 Sep;12(9). doi: 10.1038/ncb2094.

Abstract

Cell-free reconstitution of membrane traffic reactions and the morphological characterization of membrane intermediates that accumulate under these conditions have helped to elucidate the physical and molecular mechanisms involved in membrane transport. To gain a better understanding of endocytosis, we have reconstituted vesicle budding and fission from isolated plasma membrane sheets and imaged these events. Electron and fluorescence microscopy, including subdiffraction-limit imaging by stochastic optical reconstruction microscopy (STORM), revealed F-BAR (FBP17) domain coated tubules nucleated by clathrin-coated buds when fission was blocked by GTPgammaS. Triggering fission by replacing GTPgammaS with GTP led not only to separation of clathrin-coated buds, but also to vesicle formation by fragmentation of the tubules. These results suggest a functional link between FBP17-dependent membrane tubulation and clathrin-dependent budding. They also show that clathrin spatially directs plasma membrane invaginations that lead to the generation of endocytic vesicles larger than those enclosed by the coat.

PMID:
20729836
PMCID:
PMC3338250
DOI:
10.1038/ncb2094
[Indexed for MEDLINE]
Free PMC Article

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