Format

Send to

Choose Destination
See comment in PubMed Commons below
J Biol Chem. 2010 Oct 29;285(44):34240-9. doi: 10.1074/jbc.M110.159152. Epub 2010 Aug 21.

Chemoenzymatic design of heparan sulfate oligosaccharides.

Author information

1
Division of Medicinal Chemistry and Natural Products, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina 27599, USA.

Abstract

Heparan sulfate is a sulfated glycan that exhibits essential physiological functions. Interrogation of the specificity of heparan sulfate-mediated activities demands a library of structurally defined oligosaccharides. Chemical synthesis of large heparan sulfate oligosaccharides remains challenging. We report the synthesis of oligosaccharides with different sulfation patterns and sizes from a disaccharide building block using glycosyltransferases, heparan sulfate C(5)-epimerase, and sulfotransferases. This method offers a generic approach to prepare heparan sulfate oligosaccharides possessing predictable structures.

PMID:
20729556
PMCID:
PMC2962522
DOI:
10.1074/jbc.M110.159152
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center