Format

Send to

Choose Destination
J Ethnopharmacol. 2010 Oct 28;132(1):359-61. doi: 10.1016/j.jep.2010.08.018. Epub 2010 Aug 17.

The effect of Tulbaghia violacea extracts on testosterone secretion by testicular cell cultures.

Author information

1
Department of Medical Biosciences, University of the Western Cape, Private Bag X17, Modderdam Road, Bellville 7535, South Africa.

Abstract

AIM OF THE STUDY:

This study aimed to determine the effect of Tulbaghia violacea Harv. on the male reproductive system in vitro by using testicular cell cultures. Tulbaghia violacea is a plant species indigenous to southern Africa and is used locally as a herbal remedy/medicine to treat several ailments.

MATERIALS AND METHODS:

A 50% ethanol extract of Tulbaghia violacea was prepared. Three-month old male Balb/C mice were sacrificed and testicular cell cultures were prepared. Cells were then treated with varying concentrations of the Tulbaghia violacea ethanol extract (with/without Luteinizing hormone (LH)-treatment) and incubated for 4 h. Hormone production and cell viability were evaluated.

RESULTS:

Treatment of cells with Tulbaghia violacea (312.5-5000 μg ml(-1)) significantly increased (P<0.05) LH-induced testosterone production as compared to vehicle-treated control (DMSO) whereas cells without LH-treatment showed no significant change in testosterone concentrations. No significant effect on cell viability was observed at all concentrations tested.

CONCLUSIONS:

The data presented shows that Tulbaghia violacea has androgenic properties. Further studies are warranted to determine and clarify the exact mechanisms involved.

PMID:
20723589
DOI:
10.1016/j.jep.2010.08.018
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center