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Arch Virol. 2010 Nov;155(11):1855-8. doi: 10.1007/s00705-010-0768-z. Epub 2010 Aug 19.

A comparison of methods for purification and concentration of norovirus GII-4 capsid virus-like particles.

Author information

1
Vaccine Research Center, University of Tampere Medical School, Finland. lllete@uta.fi

Abstract

Noroviruses (NoVs) are one of the leading causes of acute gastroenteritis worldwide. NoV GII-4 VP1 protein was expressed in a recombinant baculovirus system using Sf9 insect cells. Several methods for purification and concentration of virus-like particles (VLPs) were evaluated. Electron microscopy (EM) and histo-blood group antigen (HBGA) binding assays showed that repeated sucrose gradient purification followed by ultrafiltration resulted in intact VLPs with excellent binding to H type 3 antigens. VLPs were stable for at least 12 months at 4°C, and up to 7 days at ambient temperature. These findings indicate that this method yielded stable and high-quality VLPs.

PMID:
20721592
PMCID:
PMC2970802
DOI:
10.1007/s00705-010-0768-z
[Indexed for MEDLINE]
Free PMC Article

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