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J Am Chem Soc. 2010 Dec 8;132(48):17053-5. doi: 10.1021/ja104204z. Epub 2010 Aug 17.

Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C20BAS bolalipid membrane vesicles.

Author information

1
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907, USA.

Abstract

Ni(2+):NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his6 gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is compatible with the hydrophobic domain of the gp10 assemblies.

PMID:
20715817
PMCID:
PMC2995011
DOI:
10.1021/ja104204z
[Indexed for MEDLINE]
Free PMC Article

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