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J Lipid Res. 2010 Nov;51(11):3091-102. doi: 10.1194/jlr.R008730. Epub 2010 Aug 16.

Shedding new light on lipid biology with coherent anti-Stokes Raman scattering microscopy.

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  • 1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.

Abstract

Despite the ubiquitous roles of lipids in biology, the detection of lipids has relied on invasive techniques, population measurements, or nonspecific labeling. Such difficulties can be circumvented by a label-free imaging technique known as coherent anti-Stokes Raman (CARS) microscopy, which is capable of chemically selective, highly sensitive, and high-speed imaging of lipid-rich structures with submicron three-dimensional spatial resolution. We review the broad applications of CARS microscopy to studies of lipid biology in cell cultures, tissue biopsies, and model organisms. Recent technical advances, limitations of the technique, and perspectives are discussed.

PMID:
20713649
PMCID:
PMC2952550
DOI:
10.1194/jlr.R008730
[PubMed - indexed for MEDLINE]
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