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Indian J Pharmacol. 2010 Apr;42(2):99-104. doi: 10.4103/0253-7613.64500.

In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves.

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1
Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576 104, India.

Abstract

OBJECTIVE:

To investigate the total alkaloid fraction of the methanol extract of leaves of Hygrophila auriculata for its hepatoprotective activity against CCl4-induced toxicity in freshly isolated rat hepatocytes, HepG2 cells, and animal models.

MATERIALS AND METHODS:

Mature leaves of H. auriculata were collected, authenticated, and subjected to methanolic extraction followed by isolation of total alkaloid fraction. Freshly isolated rat hepatocytes were exposed to CCl4 (1%) along with/without various concentrations of the total alkaloid fraction (80-40 microg/ml). Protection of human liver-derived HepG2 cells against CCl4-induced damage was determined by the MTT assay. Twenty-four healthy Wistar albino rats (150-200 g) of either sex were used for the in vivo investigations. Liver damage was induced by administration of 30% CCl4 suspended in olive oil (1 ml/kg body weight, i.p).

RESULTS:

The antihepatotoxic effect of the total alkaloid fraction was observed in freshly isolated rat hepatocytes at very low concentrations (80-40 microg/ml). A dose-dependent increase in the percentage viability was observed when CCl4-exposed HepG2 cells were treated with different concentrations of the total alkaloid fraction. Its in vivo hepatoprotective effect at 80 mg/kg body weight was comparable with that of the standard Silymarin at 250 mg/kg body weight.

CONCLUSION:

The total alkaloid fraction was able to normalize the biochemical levels which were altered due to CCl4 intoxication.

KEYWORDS:

HepG2; Hygrophila auriculata; hepatocytes; hepatoprotective; total alkaloidsIntroduction

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