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J Clin Pathol. 2010 Aug;63(8):702-7. doi: 10.1136/jcp.2010.078717.

Expression of intestinal MUC17 membrane-bound mucin in inflammatory and neoplastic diseases of the colon.

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1
Department of Biochemistry and Molecular Biology, University of Nebraska, Omaha, Nebraska, USA.

Abstract

AIM:

To determine the cellular location and expression of MUC17 mucin in specimens of normal, inflamed and neoplastic colon.

METHODS:

Immunohistochemical analysis of human surgical resection specimens (n=106) was performed with a specific antibody to the MUC17 apomucin protein. A semi-quantitative scoring system was used to measure MUC17 expression. In various colon cancer cell lines, the MUC17 expression was examined by immunoblot analysis and normal RT-PCR.

RESULTS:

MUC17 was highly expressed on the surface epithelium and crypts of colonic mucosa. In contrast, the expression of MUC17 was significantly decreased in colonic mucosa of chronic ulcerative colitis (p<0.0001) and ischaemic colitis (p=0.003). Similarly, MUC17 expression was decreased in hyperplastic polyps (p=0.0003), tubular and tubulovillous adenomas (p<0.0001) and colon cancers (p<0.0001). Furthermore, of eight different colon cancer cell lines, MUC17 expression was only detected in LS174T and LS180 cells.

CONCLUSION:

Results indicate that the potential protective effects of this membrane-bound mucin are primarily or secondarily diminished in inflammatory and neoplastic conditions. Further research is needed to determine the specific role of MUC17 in the pathogenesis of these conditions.

PMID:
20702471
PMCID:
PMC2997570
DOI:
10.1136/jcp.2010.078717
[Indexed for MEDLINE]
Free PMC Article
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