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Toxicol In Vitro. 1990;4(4-5):657-9.

Transformation of ras transfected BALB 3T3 clone (Bhas 42) by promoters: Application for screening and specificity of promoters.

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1
Laboratory of Cell Toxicology, Department of Cell Biology, Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano, Kanagawa 257, Japan.

Abstract

BALB 3T3 cells transfected by v-Ha-ras (Bhas 42 clone) were found to be sensitive to contact inhibition, but were susceptible to drastic transformation by 12-O-tetradecanoylphorbol-13-acetate. These results indicate that Bhas 42 cells are the initiated cells in the two-stage transformation process. By plating Bhas 42 cells together with BALB 3T3 cells followed by treatment with known promoters, a transformation assay system was established for the detection of tumour promoters. The Bhas 42 system showed advantages over conventional chemically induced transformation assays at several points, but some promoters did not induce transformation of Bhas 42 cells.

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