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Cancer Epidemiol Biomarkers Prev. 2010 Sep;19(9):2262-71. doi: 10.1158/1055-9965.EPI-10-0126. Epub 2010 Aug 10.

Rapid mass spectrometric metabolic profiling of blood sera detects ovarian cancer with high accuracy.

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1
Georgia Institute of Technology, Atlanta, Georgia 30332-0363, USA.

Abstract

BACKGROUND:

Ovarian cancer diagnosis is problematic because the disease is typically asymptomatic, especially at the early stages of progression and/or recurrence. We report here the integration of a new mass spectrometric technology with a novel support vector machine computational method for use in cancer diagnostics, and describe the application of the method to ovarian cancer.

METHODS:

We coupled a high-throughput ambient ionization technique for mass spectrometry (direct analysis in real-time mass spectrometry) to profile relative metabolite levels in sera from 44 women diagnosed with serous papillary ovarian cancer (stages I-IV) and 50 healthy women or women with benign conditions. The profiles were input to a customized functional support vector machine-based machine-learning algorithm for diagnostic classification. Performance was evaluated through a 64-30 split validation test and with a stringent series of leave-one-out cross-validations.

RESULTS:

The assay distinguished between the cancer and control groups with an unprecedented 99% to 100% accuracy (100% sensitivity and 100% specificity by the 64-30 split validation test; 100% sensitivity and 98% specificity by leave-one-out cross-validations).

CONCLUSION:

The method has significant clinical potential as a cancer diagnostic tool. Because of the extremely low prevalence of ovarian cancer in the general population (approximately 0.04%), extensive prospective testing will be required to evaluate the test's potential utility in general screening applications. However, more immediate applications might be as a diagnostic tool in higher-risk groups or to monitor cancer recurrence after therapeutic treatment.

IMPACT:

The ability to accurately and inexpensively diagnose ovarian cancer will have a significant positive effect on ovarian cancer treatment and outcome.

PMID:
20699376
DOI:
10.1158/1055-9965.EPI-10-0126
[Indexed for MEDLINE]
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