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J Exp Clin Cancer Res. 2010 Aug 10;29:108. doi: 10.1186/1756-9966-29-108.

Parthenolide induces proliferation inhibition and apoptosis of pancreatic cancer cells in vitro.

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1
Department of General Surgery, Sir Run Run Shaw Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, PR China.

Abstract

BACKGROUND:

To explore the anti-tumor effects of parthenolide in human pancreatic cancer.

METHODS:

BxPC-3 cell, a human pancreatic cancer, was treated with parthenolide at different concentrations. The MTT assay was used to analyze cell viability. Flow cytometry and DNA fragmentation analysis were applied to evaluate apoptosis after parthenolide treatment. The wound closure and cell invasion assay were also employed in the study. Western blotting was used to demonstrate Bad, Bcl-2, Bax, caspase-9 and pro-caspase-3 expression.

RESULTS:

The MTT assay indicated that the pancreatic cancer growth could be dose-dependently inhibited by parthenoolide. This phenomenon was confirmed by flow cytometry and DNA fragmentation analysis. The wound closure assay and cell invasion assay showed that BxPC-3 cell was significantly suppressed by parthenolide at 7.5 microM and 15 microM. Western Blotting demonstrated the Bcl-2 and pro-caspase-3 were down-regulated while the Bax and caspase-9 were up-regulated. No alteration in Bad expression was found after treatment.

CONCLUSIONS:

The parthenolide can inhibit the cell growth, migration, and induce the apoptosis in human pancreatic cancer. These findings may provide a novel approach for pancreatic cancer treatment.

PMID:
20698986
PMCID:
PMC2924280
DOI:
10.1186/1756-9966-29-108
[Indexed for MEDLINE]
Free PMC Article
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