Format

Send to

Choose Destination
Methods Mol Biol. 2010;647:77-93. doi: 10.1007/978-1-60761-738-9_4.

Subnuclear localization and intranuclear trafficking of transcription factors.

Author information

1
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA.

Abstract

Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

PMID:
20694661
PMCID:
PMC3607955
DOI:
10.1007/978-1-60761-738-9_4
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center