Format

Send to

Choose Destination
See comment in PubMed Commons below
Biomaterials. 2010 Nov;31(31):8097-105. doi: 10.1016/j.biomaterials.2010.07.015. Epub 2010 Aug 7.

Polyion complex stability and gene silencing efficiency with a siRNA-grafted polymer delivery system.

Author information

1
Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.

Abstract

An siRNA-grafted polymer through disulfide linkage was prepared to improve the physicochemical properties and transfection efficacies of the polyion complexes (PICs) as a nanocarrier of siRNA. The siRNA-grafted polymer formed stable PICs due to its larger numbers and higher density of anionic charges compared with monomeric siRNA, leading to effective internalization by cultured cells. Following the endosomal escape of the PIC, the disulfide linkage of the siRNA-grafted polymer allowed efficient siRNA release from the PIC under intracellular reductive conditions. Consequently, the PIC from the siRNA-grafted polymer showed a potent gene silencing effect without cytotoxicity or immunogenicity, demonstrating a promising feature of the siRNA-grafted polymer to construct the PIC-based nanocarrier for in vivo siRNA delivery.

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center