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BMC Dev Biol. 2010 Aug 6;10:84. doi: 10.1186/1471-213X-10-84.

Involvement of the Reck tumor suppressor protein in maternal and embryonic vascular remodeling in mice.

Author information

1
Department of Molecular Oncology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8501, Japan.

Abstract

BACKGROUND:

Developmental angiogenesis proceeds through multiple morphogenetic events including sprouting, intussusception, and pruning. Mice lacking the membrane-anchored metalloproteinase regulator Reck die in utero around embryonic day 10.5 with halted vascular development; however, the mechanisms by which this phenotype arises remain unclear.

RESULTS:

We found that Reck is abundantly expressed in the cells associated with blood vessels undergoing angiogenesis or remodelling in the uteri of pregnant female mice. Some of the Reck-positive vessels show morphological features consistent with non-sprouting angiogenesis. Treatment with a vector expressing a small hairpin RNA against Reck severely disrupts the formation of blood vessels with a compact, round lumen. Similar defects were found in the vasculature of Reck-deficient or Reck conditional knockout embryos.

CONCLUSIONS:

Our findings implicate Reck in vascular remodeling, possibly through non-sprouting angiogenesis, in both maternal and embyonic tissues.

PMID:
20691046
PMCID:
PMC2923112
DOI:
10.1186/1471-213X-10-84
[Indexed for MEDLINE]
Free PMC Article

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