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J Struct Funct Genomics. 2010 Sep;11(3):191-9. doi: 10.1007/s10969-010-9094-7. Epub 2010 Aug 6.

The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins.

Author information

1
New York Structural Biology Center, New York, 10027, USA.

Abstract

The New York Consortium on Membrane Protein Structure (NYCOMPS) was formed to accelerate the acquisition of structural information on membrane proteins by applying a structural genomics approach. NYCOMPS comprises a bioinformatics group, a centralized facility operating a high-throughput cloning and screening pipeline, a set of associated wet labs that perform high-level protein production and structure determination by x-ray crystallography and NMR, and a set of investigators focused on methods development. In the first three years of operation, the NYCOMPS pipeline has so far produced and screened 7,250 expression constructs for 8,045 target proteins. Approximately 600 of these verified targets were scaled up to levels required for structural studies, so far yielding 24 membrane protein crystals. Here we describe the overall structure of NYCOMPS and provide details on the high-throughput pipeline.

PMID:
20690043
PMCID:
PMC3099345
DOI:
10.1007/s10969-010-9094-7
[Indexed for MEDLINE]
Free PMC Article

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