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Circ Res. 2010 Oct 1;107(7):903-12. doi: 10.1161/CIRCRESAHA.110.220772. Epub 2010 Aug 5.

Receptor-independent cardiac protein kinase Calpha activation by calpain-mediated truncation of regulatory domains.

Author information

1
Washington University Center for Pharmacogenomics, St Louis, MO 63110, USA.

Abstract

RATIONALE:

Protein kinase (PK)Cs and calpain cysteine proteases are highly expressed in myocardium. Ischemia produces calcium overload that activates calpains and conventional PKCs. However, calpains can proteolytically process PKCs, and the potential in vivo consequences of this interaction are unknown.

OBJECTIVE:

To determine the biochemical and pathophysiological consequences of calpain-mediated cardiac PKCα proteolysis.

METHODS AND RESULTS:

Isolated mouse hearts subjected to global ischemia/reperfusion demonstrated cleavage of PKCα. Calpain 1 overexpression was not sufficient to produce PKCα cleavage in normal hearts, but ischemia-induced myocardial PKCα cleavage and myocardial injury were greatly increased by cardiac-specific expression of calpain 1. In contrast, calpain 1 gene ablation or inhibition with calpastatin prevented ischemia/reperfusion induced PKCα cleavage; infarct size was decreased and ventricular function enhanced in infarcted calpain 1 knockout hearts. To determine consequences of PKCα fragmentation on myocardial protein phosphorylation, transgenic mice were created conditionally expressing full-length PKCα or its N-terminal and C-terminal calpain 1 cleavage fragments. Two-dimensional mapping of ventricular protein extracts showed a distinct PKCα phosphorylation profile that was exaggerated and distorted in hearts expressing the PKCα C-terminal fragment. MALDI mass spectroscopy revealed hyperphosphorylation of myosin-binding protein C and phosphorylation of atypical substrates by the PKCα C-terminal fragment. Expression of parent PKCα produced a mild cardiomyopathy, whereas myocardial expression of the C-terminal PKCα fragment induced a disproportionately severe, rapidly lethal cardiomyopathy.

CONCLUSIONS:

Proteolytic processing of PKCα by calcium-activated calpain activates pathological cardiac signaling through generation of an unregulated and/or mistargeted kinase. Production of the PKCα C-terminal fragment in ischemic hearts occurs via a receptor-independent mechanism.

PMID:
20689063
PMCID:
PMC2948630
DOI:
10.1161/CIRCRESAHA.110.220772
[Indexed for MEDLINE]
Free PMC Article

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