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Nucleic Acids Res. 2010 Oct;38(19):e181. doi: 10.1093/nar/gkq684. Epub 2010 Aug 4.

Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA.

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1
Department of Biology, Center for Integrated Protein Science Munich, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany.

Abstract

The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.

PMID:
20685817
PMCID:
PMC2965258
DOI:
10.1093/nar/gkq684
[Indexed for MEDLINE]
Free PMC Article
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