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Genesis. 2010 Oct 1;48(10):607-11. doi: 10.1002/dvg.20661.

Characterization of midgut stem cell- and enteroblast-specific Gal4 lines in drosophila.

Author information

1
Mouse Cancer Genetics Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland, USA. zengx2@mail.nih.gov

Abstract

The homeostasis of Drosophila midgut is maintained by multipotent intestinal stem cells (ISCs), each of which gives rise to a new ISC and an immature daughter cell, enteroblast (EB), after one asymmetric cell division. In Drosophila, the Gal4-UAS system is widely used to manipulate gene expression in a tissue- or cell-specific manner, but in Drosophila midgut, there are no ISC- or EB-specific Gal4 lines available. Here we report the generation and characterization of Dl-Gal4 and Su(H)GBE-Gal4 lines, which are expressed specifically in the ISCs and EBs separately. Additionally, we demonstrate that Dl-Gal4 and Su(H)GBE-Gal4 are expressed in adult midgut progenitors (AMPs) and niche peripheral cells (PCs) separately in larval midgut. These two Gal4 lines will serve as invaluable tools for navigating ISC behaviors.

PMID:
20681020
PMCID:
PMC2958251
DOI:
10.1002/dvg.20661
[Indexed for MEDLINE]
Free PMC Article

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