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Methods Mol Biol. 2010;649:163-82. doi: 10.1007/978-1-60761-753-2_10.

Remodeling genomes with artificial transcription factors (ATFs).

Author information

1
Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Abstract

Chromatin structure plays a pivotal role in defining which regions of the genome are accessible for effective transcription. Chromatin-remodeling agents are able to relax this structure, facilitating the access of transcription factors into the DNA. Herein, we describe a new method, which combines artificial transcription factors (ATFs) and chromatin-remodeling agents to specifically reactivate silenced regions of the genome and reprogram cellular phenotypes.

PMID:
20680834
DOI:
10.1007/978-1-60761-753-2_10
[Indexed for MEDLINE]

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