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Vet Microbiol. 2011 Jan 27;147(3-4):226-30. doi: 10.1016/j.vetmic.2010.06.026. Epub 2010 Jul 1.

Detection and genotyping of GB virus-C in dromedary camels in the United Arab Emirates.

Author information

1
College of Health Sciences, Department of Medical Laboratory Technology, University of Sharjah, Sharjah, United Arab Emirates. abuodeh@sharjah.ac.ae

Abstract

GB virus-C/hepatitis G virus (GBV-C/HGV), collectively known as GBV-C, is widely spread and was discovered while searching for a causative agent for non-A-E hepatitis. The aim of this study was to determine the rate of infection and genotypic characteristics of GBV-C in Arabian camels in the United Arab Emirates (UAE). A total of 22 blood and 8 milk samples from healthy camels were screened using RT-PCR/nested PCR of the 5'-untranslated region (UTR). Phylogenetic analysis was conducted by sequencing the UTR region of randomly picked clones. The results obtained showed that the rate of GBV-C infection in camels was 18.2% (4 out of 22). All camels milk samples tested negative. Sequence analysis of the 5'-UTR using isolates from the 4 camels revealed the prevalence of the European/North American genotype 2 when compared to the 6 reference genotypes in GenBank.

PMID:
20674194
DOI:
10.1016/j.vetmic.2010.06.026
[Indexed for MEDLINE]

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