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N Biotechnol. 2011 Apr 30;28(3):272-6. doi: 10.1016/j.nbt.2010.07.014. Epub 2010 Aug 4.

Cell-free expression and labeling strategies for a new decade in solid-state NMR.

Author information

1
UMR 7099, CNRS and Université Paris Diderot, IBPC, 13 rue Pierre et Marie Curie, F-75005 Paris, France.

Abstract

Although solid-state NMR and cell-free expression have recently become standard methods in biology, the combination of the two is still at a very early stage of development. In this article, we will explore several approaches by which cell-free expression could help solid-state NMR in its quest for biomolecular structure and mechanism elucidation. Far from being just another structure determination technique, this quest is motivated by the unique possibility of using solid-state NMR to determine the high resolution structure of a membrane protein within its native environment, the lipid membrane. We will examine the specific sample preparation requirements that such a goal imposes and how cell-free expression can play a key role in such a protocol.

PMID:
20667518
DOI:
10.1016/j.nbt.2010.07.014
[Indexed for MEDLINE]

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