Format

Send to

Choose Destination
See comment in PubMed Commons below
Biotechnol J. 2010 Jul;5(7):716-25. doi: 10.1002/biot.201000171.

Microbial 1-butanol production: Identification of non-native production routes and in silico engineering interventions.

Author information

1
Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.

Abstract

The potential of engineering microorganisms with non-native pathways for the synthesis of long-chain alcohols has been identified as a promising route to biofuels. We describe computationally derived predictions for assembling pathways for the production of biofuel candidate molecules and subsequent metabolic engineering modifications that optimize product yield. A graph-based algorithm illustrates that, by culling information from BRENDA and KEGG databases, all possible pathways that link the target product with metabolites present in the production host are identified. Subsequently, we apply our recent OptForce procedure to pinpoint reaction modifications that force the imposed product yield in Escherichia coli. We demonstrate this procedure by suggesting new pathways and genetic interventions for the overproduction of 1-butanol using the metabolic model for Escherichia coli. The graph-based search method recapitulates all recent discoveries based on the 2-ketovaline intermediate and hydroxybutyryl-CoA but also pinpoints one novel pathway through thiobutanoate intermediate that to the best of our knowledge has not been explored before.

PMID:
20665644
DOI:
10.1002/biot.201000171
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center