A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens

Biotechnol Lett. 2010 Dec;32(12):1789-96. doi: 10.1007/s10529-010-0359-9. Epub 2010 Jul 28.

Abstract

A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase activation in Huh7-PPRE-Luc cells and caused adipogenic differentiation of 3T3-L1 cells. This recombinant Huh7-PPRE-Luc cell line would be useful for screening potential environmental obesogens with PPAR activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipogenesis
  • Biological Assay / methods*
  • Cell Differentiation
  • Cell Line
  • Dose-Response Relationship, Drug
  • Environmental Pollutants / analysis*
  • Hepatocytes / metabolism
  • Humans
  • Luciferases / biosynthesis*
  • Peroxisome Proliferators / metabolism*
  • Response Elements*

Substances

  • Environmental Pollutants
  • Peroxisome Proliferators
  • Luciferases