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J Bacteriol. 1991 Jul;173(14):4537-9.

The hemimethylated replication origin of Escherichia coli can be initiated in vitro.

Author information

1
Department of Biophysics, Norwegian Radium Hospital, Oslo.

Abstract

Unmethylated, fully methylated, and hemimethylated oriC-containing plasmids were assayed as substrates for DNA replication in vitro by using a system reconstituted with pure proteins. In contrast to the in vivo situation, all three substrates were initiated efficiently; the fully methylated plasmid was about twice as active as the other two.

PMID:
2066345
PMCID:
PMC208120
[Indexed for MEDLINE]
Free PMC Article

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