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Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14345-50. doi: 10.1073/pnas.1000044107. Epub 2010 Jul 26.

Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice.

Author information

1
Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.

Erratum in

  • Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14514.

Abstract

Attempts to treat congenital protein deficiencies using bone marrow-derived cells have been reported. These efforts have been based on the concepts of stem cell plasticity. However, it is considered more difficult to restore structural proteins than to restore secretory enzymes. This study aims to clarify whether bone marrow transplantation (BMT) treatment can rescue epidermolysis bullosa (EB) caused by defects in keratinocyte structural proteins. BMT treatment of adult collagen XVII (Col17) knockout mice induced donor-derived keratinocytes and Col17 expression associated with the recovery of hemidesmosomal structure and better skin manifestations, as well improving the survival rate. Both hematopoietic and mesenchymal stem cells have the potential to produce Col17 in the BMT treatment model. Furthermore, human cord blood CD34(+) cells also differentiated into keratinocytes and expressed human skin component proteins in transplanted immunocompromised (NOD/SCID/gamma(c)(null)) mice. The current conventional BMT techniques have significant potential as a systemic therapeutic approach for the treatment of human EB.

PMID:
20660747
PMCID:
PMC2922560
DOI:
10.1073/pnas.1000044107
[Indexed for MEDLINE]
Free PMC Article

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