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Appl Environ Microbiol. 2010 Sep;76(18):6313-7. doi: 10.1128/AEM.00882-10. Epub 2010 Jul 23.

Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system.

Author information

1
Research Center for Infectious Diseases, Julius-Maximilians-University Würzburg, D-97080 Würzburg, Germany.

Abstract

Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing beta serine recombinase acting on six recognition sequences (beta-rec/six) in a fungal host, the human pathogen Aspergillus fumigatus, and its use in establishing a self-excising resistance marker cassette for serial gene replacement.

PMID:
20656854
PMCID:
PMC2937505
DOI:
10.1128/AEM.00882-10
[Indexed for MEDLINE]
Free PMC Article

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