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Cytometry A. 2010 Aug;77(8):776-82. doi: 10.1002/cyto.a.20925.

Fluorogen-activating proteins as biosensors of cell-surface proteins in living cells.

Author information

1
Department of Biological Sciences, Technology Center for Networks and Pathways, Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.

Abstract

This study explores the general utility of a new class of biosensor that allows one to selectively visualize molecules of a chosen membrane protein that are at the cell surface. These biosensors make use of recently described bipartite fluoromodules comprised of a fluorogen-activating protein (FAP) and a small molecule (fluorogen) whose fluorescence increases dramatically when noncovalently bound by the FAP (Szent-Gyorgyi et al., Nat Biotechnol 2010;00:000-000).

PMID:
20653017
PMCID:
PMC2945705
DOI:
10.1002/cyto.a.20925
[Indexed for MEDLINE]
Free PMC Article
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