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Mediators Inflamm. 2010;2010:745724. doi: 10.1155/2010/745724. Epub 2010 Jun 21.

High-mobility group box-1 protein serum levels do not reflect monocytic function in patients with sepsis-induced immunosuppression.

Author information

1
Department of Medical Immunology, Charité University Medicine, Campus Mitte, 10117 Berlin, Germany.

Abstract

BACKGROUND:

High-mobility group box-1 (HMGB-1) protein is released during "late sepsis" by activated monocytes. We investigated whether systemic HMGB-1 levels are associated with indices of monocytic activation/function in patients with sepsis-induced immunosuppression.

METHODOLOGY:

36 patients (31 male, 64 +/- 14 years) with severe sepsis/septic shock and monocytic deactivation (reduced mHLA-DR expression and TNF-alpha release) were assessed in a subanalysis of a placebo-controlled immunostimulatory trial using GM-CSF. HMGB-1 levels were assessed over a 9-day treatment interval. Data were compared to standardized biomarkers of monocytic immunity (mHLA-DR expression, TNF-alpha release).

PRINCIPLE FINDINGS:

HMGB-1 levels were enhanced in sepsis but did not differ between treatment and placebo groups at baseline (14.6 +/- 13.5 versus 12.5 +/- 11.5 ng/ml, P = .62). When compared to controls, HMGB-1 level increased transiently in treated patients at day 5 (27.8 +/- 21.7 versus 11.0 +/- 14.9, P = .01). Between group differences were not noted at any other point of assessment. HMGB-1 levels were not associated with markers of monocytic function or clinical disease severity.

CONCLUSIONS:

GM-CSF treatment for sepsis-induced immunosuppression induces a moderate but only transient increase in systemic HMGB-1 levels. HMGB-1 levels should not be used for monitoring of monocytic function in immunostimulatory trials as they do not adequately portray contemporary changes in monocytic immunity.

PMID:
20652004
PMCID:
PMC2905954
DOI:
10.1155/2010/745724
[Indexed for MEDLINE]
Free PMC Article

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