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Anticancer Res. 2010 Jun;30(6):2033-40.

Protective effects of the immunopotentiator from Pantoea agglomerans 1 on chemotherapeutic agent-induced macrophage growth inhibition.

Author information

1
Laboratory of Veterinary Physiology, Division of Animal Life Science, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-0859, Japan.

Abstract

BACKGROUND:

The immunopotentiator from Pantoea agglomerans 1 (IP-PA1) is an edible lipopolysaccharide (LPS) derived from symbiotic bacteria found in crops. IP-PA1 is known to ameliorate chemotherapy-induced immunosuppression; therefore, its macrophage-activating effect in the presence of chemotherapeutic agents was evaluated.

MATERIALS AND METHODS:

Nuclear factor-kappaB (NF-kappaB) activation in IP-PA1-treated RAW264 and J774.1 cells was examined using Western blot analyses; Griess assay and ELISA were used to examine the production of nitric oxide and tumour necrosis factor alpha, respectively. The expression of apoptosis-related proteins was also assessed using Western blot analyses. The effect of IP-PA1 on doxorubicin-induced apoptosis was analyzed by flow cytometry after annexin-V staining. The growth of macrophages treated with chemotherapeutic agents and IP-PA1 was analyzed using an MTT assay.

RESULTS:

IP-PA1 activated NF-kappaB and ameliorated chemotherapy induced growth inhibition in the cells.

CONCLUSION:

IP-PA1 is an edible drug that can potentially support chemotherapy by ameliorating chemotherapy-induced immunosuppression.

PMID:
20651348
[Indexed for MEDLINE]

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