Format

Send to

Choose Destination
J Cell Sci. 2010 Aug 15;123(Pt 16):2725-32. doi: 10.1242/jcs.062299. Epub 2010 Jul 20.

Manipulating signal delivery - plasma-membrane ERK activation in aPKC-dependent migration.

Author information

1
Protein Phosphorylation Laboratory, Cancer Research UK, London Research Institute, London, WC2A 3PX, UK.

Abstract

Members of the PKC superfamily have been implicated in various migratory models and in particular in spatially restricted processes. However, defining the precise local events that underlie the PKC-dependent processes is constrained by the unspecific nature of interventions. Here we address this problem in relation to atypical PKC (aPKC) action, which in conjunction with the exocyst complex controls the polarised delivery of promigratory signals. A drug-dependent recruitment approach was employed to manipulate the local recruitment of signals to the leading edge of migrating cells, under conditions where the aPKC-exocyst control is globally abrogated. We found that activation of ERK but not JNK at focal adhesions recovers the majority of the migratory loss attributed to ERK action, demonstrating a necessary role for active plasma membrane ERK in the downstream signalling of aPKC-dependent migration. The data further show that restored focal adhesion dynamics are a contributing mechanism through which localized ERK activity influences this aPKC-exocyst-dependent migration.

PMID:
20647370
DOI:
10.1242/jcs.062299
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center