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Biochem Biophys Res Commun. 2010 Aug 13;399(1):84-90. doi: 10.1016/j.bbrc.2010.07.046. Epub 2010 Jul 17.

ERK5 pathway regulates the phosphorylation of tumour suppressor hDlg during mitosis.

Author information

1
Departamento de Inmunología y Oncología, Centro Nacional de Biotecnología-CSIC, Campus de Cantoblanco-UAM, Madrid, Spain.

Abstract

Human disc-large (hDlg) is a scaffold protein critical for the maintenance of cell polarity and adhesion. hDlg is thought to be a tumour suppressor that regulates the cell cycle and proliferation. However, the mechanism and pathways involved in hDlg regulation during these processes is still unclear. Here we report that hDlg is phosphorylated during mitosis, and we establish the identity of at least three residues phosphorylated in hDlg; some are previously unreported. Phosphorylation affects hDlg localisation excluding it from the contact point between the two daughter cells. Our results reveal a previously unreported pathway for hDlg phosphorylation in mitosis and show that ERK5 pathway mediates hDlg cell cycle dependent phosphorylation. This is likely to have important implications in the correct timely mitotic entry and mitosis progression.

PMID:
20643107
DOI:
10.1016/j.bbrc.2010.07.046
[Indexed for MEDLINE]

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