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Nat Methods. 2010 Aug;7(8):631-3. doi: 10.1038/nmeth.1482. Epub 2010 Jul 18.

Single-allele analysis of transcription kinetics in living mammalian cells.

Author information

1
The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar-Ilan University, Ramat Gan, Israel.

Abstract

We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.

PMID:
20639867
DOI:
10.1038/nmeth.1482
[Indexed for MEDLINE]

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