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J Bacteriol. 2010 Sep;192(18):4694-700. doi: 10.1128/JB.00570-10. Epub 2010 Jul 16.

Separate DNA Pol II- and Pol IV-dependent pathways of stress-induced mutation during double-strand-break repair in Escherichia coli are controlled by RpoS.

Author information

1
Department of Molecular and Human Genetics, Baylor College of Medicine,Baylor College of Medicine, Houston, Texas 77030-3411, USA.

Abstract

Previous work showed that about 85% of stress-induced mutations associated with DNA double-strand break repair in carbon-starved Escherichia coli result from error-prone DNA polymerase IV (Pol IV) (DinB) and that the mutagenesis is controlled by the RpoS stress response, which upregulates dinB. We report that the remaining mutagenesis requires high-fidelity Pol II, and that this component also requires RpoS. The results identify a second DNA polymerase contributing to stress-induced mutagenesis and show that RpoS promotes mutagenesis by more than the simple upregulation of dinB.

PMID:
20639336
PMCID:
PMC2937414
DOI:
10.1128/JB.00570-10
[Indexed for MEDLINE]
Free PMC Article
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